MRC Elsie Widdowson Laboratory

The NDNS Biorepository

Repository and residual biological samples collected from the different surveys have been stored to be available for further research, providing the opportunity for data linkage at the individual level.

The collection contains samples of urine, plasma, serum and whole blood from the current NDNS RP, urinary sodium surveys 2011-15 in England, Scotland and Northern Ireland, the Diet and Nutrition Survey of Infants and Young Children (DNSIYC) and past NDNS.

The NDNS Biorepository forms a key part of the NDNS Bioresource along with access to NDNS RP/DNSIYC participants for follow-up research and NHS data linkage.

What’s in the NDNS Biorepository collection?

The Biorepository contains urine, plasma, serum and whole blood samples. The tables below provide a provisional indication of the different surveys, dates of sample collection, age groups and sample size and approximate numbers of urine and blood samples. Sample amounts and types vary between the different surveys and for some of the earlier surveys, records are not fully inventoried and catalogued. If you would like further information about a specific age group, survey year or sample type please email: ndns.bioresource@phe.gov.uk for more specific information. Charges may apply.

 

Survey Survey year Dates of sample collection Population age Dietary and other core variables data (n) Urine samples (approx. n)a Blood samples (approx. n)b
NDNS RP Y1c 2008/09 1.5y – 18y 845 390 200
19y+ 801 470 370
NDNS RP Y2d 2008/10 1.5y – 18y 857 410 230
19y+ 812 510 450
NDNS RP Y3d 2008/11 1.5y – 18y 783 370 210
19y+ 782 430 380
NDNS RP Y4d 2008/12 1.5y – 18y 893 420 250
19y+ 1055 640 560
NDNS RP Y5e 2008/13 1.5y – 18y 572 270 160
19y+ 625 430 370
NDNS RP Y6f 2008/14 1.5y – 18y 686 460 190
19y+ 663 560 350
NDNS RP Y7f 2008/15 1.5y – 18y 650 390 150
19y+ 703 580 340
NDNS RP Y8f 2008/16 1.5y – 18y 656 400 170
19y+ 714 570 350
NDNS RP Y9f 2008/17 1.5y – 18y In progress
19y+ In progress

Urine samples for Years 1-5 were 24-hour urine collections, whereas urine samples for Years 6-9 were a single spot sample.

Due to sample volume collected and varying consents, the total number available in the repository will be lower than the number of subjects originally in the project.

c There were country boosts for Scotland and Northern Ireland for this survey year.

d There were country boosts for Scotland, Northern Ireland and Wales for this survey year.

e There was a country boost for Wales only for this survey year.

f There were country boosts for Wales and Northern Ireland for this survey year.

 

 

Survey Dates of sample collection Population age Dietary and other core variables data (n) Urine samples (approx. n) Blood samples (approx. n)a
DNSYIC 2011 4 – 18 months 2683 n/a 460
Urinary Sodium Surveys
England 2014 19y – 64y n/a 680 n/a
Scotland 2014 19y – 64y n/a 660 n/a
Northern Ireland 2014 19y – 64y n/a 600 n/a
England 2011 19y – 64y n/a 540 n/a
Past NDNS
Adults 2000-2001 19y – 64y 1724 To be catalogued 1000
Young People 1997 4y – 18y 1701 To be catalogued To be catalogued
Older Adults 1994/95 65y+ 1687 To be catalogued To be catalogued
Pre-school Children 1992/93 1.5y – 4.5y 1675 n/a To be catalogued

aDue to sample volume collected and varying consents, the total number available in the repository will be lower than the number of subjects originally in the project.

How are samples collected and stored?

NDNS RP samples include samples obtained specifically for deposit in the NDNS Biorepository, and also residual material remaining after primary sample analysis, where participant consent has been granted. For details of how the blood and urine samples are obtained and processed in the NDNS RP (2008 – ongoing) see Appendix O and Appendix T of the NDNS Results from Years 1 to 4. Biological samples from other surveys are made up from residual sample post primary analysis.

Blood samples from the surveys are stored in -80ºC in mechanical freezers at MRC Elsie Widdowson Laboratory (MRC EWL). Urine samples are stored at -20 ºC also at MRC EWL.

MRC EWL is currently the custodian for the NDNS biological sample collection. Long term storage of donated samples for the surveys is with written participant consent and NHS ethics approval.
MRC EWL holds a Human Tissue Licence from the Human Tissue Licence Authority.